Percoll-gradient separation of Leydig cells from postnatal rat testes
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چکیده
منابع مشابه
Postnatal Development of Interstitial Endocrine (leydig) Cells in Pig’s Testes – Electron Microscopy Study
The normal ultrastructure of interstitial endocrinocytes in testes of pigs aged from 1 day to 8 months was determined by electron microscopy. It was found out that these cells had similar ultrastructural traits during the entire experimental period – a very well developed agranular reticulum of tubulovesicular type and numerous mitochondria with tubulous cristae, evidence for active steroidogen...
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The isolation of pancreatic islets from collagenase-digested Wistar rat pancreas was shown by the sedimentation method at a unit gravity using Percoll solution with a density of 1.041 g/ml. The density of digested exocrine tissues was in the range of 1.013-1.041 g/ml, while that of purely isolated islets was in the narrow range of 1.066-1.075 g/ml. More than a hundred islets were obtained freel...
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The ultrastructure and developmental fate of the fetal generation of Leydig cells of the rat testis was studied from the 17th day of fetal life up to 100 days after birth. The number of fetal Leydig cells per testis was determined by light microscopic morphometric analysis of semithin plastic sections. In fetal testes (days 17-22 postconception), Leydig cells exhibited a characteristic ultrastr...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1986
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0760331